MISiS: Multi-layer antibacterial coatings for implants

The main articles are:

• Medical Devices Market in Russia
• Antibacterial drugs and materials

2022: Antibacterial nano-coating based on boron nitride and ultrafine metallized silver

Materials scientists of the University "MISIS" have created antibacterial nano-coatings against microbial and fungal pathogens that do not have characteristic negative side effects and can become a safe alternative to antibiotics for use in traumatology, surgery and implantology. Material based on boron nitride and ultrafine metallized silver or iron oxide nanoparticles has efficiency up to 99.99%. As of August 2022, the obtained samples are tested as coatings for implants. This was announced on August 9, 2022 by representatives of the MISIS University.

Scientists have created antibacterial nano-coatings based on boron nitride and ultra-dispersed metallized silver. Photo: minobrnauki.gov.ru.

According to the company, the developers emphasize that as a result of the tests, it was proved that the coating is safe for the patient's body. The difference from analogues was the minimum doses of bactericidal components and the complete absence of an antibiotic filler, which eliminates resistance.

A group of scientists from NUST MISIS, together with colleagues from the State Scientific Center for Applied Microbiology and Biotechnology, proposed a non-standard complex triple effect on infectious pathogens: the needle surface of nanoparticles physically damages the membranes of bacterial cells, then metal ions with a bactericidal effect are released, and then reactive oxygen species are formed that destroy disease-causing organisms. The enhanced bactericidal and fungicidal activity of the nano-coating is associated with the formation of a large number of reactive oxygen species: free radicals damage the walls of microorganisms, as a result of which the latter die.

We synthesized coatings consisting of boron nitride nanoparticles modified with ultrafine silver nanoparticles, or iron oxide. Boron nitride carriers have a unique spherical shape with a needle surface that allows damage and rupture of the membrane of bacterial cells upon physical contact with them. The coatings themselves release metal ions depending on the concentration. Our studies have shown that at a minimal inhibitory concentration, iron oxide nanoparticles (74 μg/cm2) effectively inhibit the growth of Gram-negative bacillus bacteria intestinal as well as Staphylococcus aureus and pneumococci within the first three hours. Coatings with silver at a minimum concentration of 12 μg/cm completely inactivate the bacteria. told Christina Gudz, one of the authors of the study, an employee of the Inorganic Nanomaterials Laboratory of MISIS University

It turned out that such a coating destroys 100% of the studied microorganisms: bacterial strains and fungus Candida parapsilosis die within a day after exposure. According to the researchers, only they can get the spherical and needle-shaped, "shaggy" form of boron nitride nanoparticles, its feature is registered in the form of Russian know-how: "A method for producing nanostructured coatings of hexagonal boron nitride with an antibacterial effect."

The next step will be to scale development as a dressing for use in traumatology and surgery. Also in the future, it is planned to conduct in-vitro studies, as of August 2022, the priority is to conduct studies on especially dangerous strains of bacteria and viruses (Vibrio cholerae, Covid-19, etc.).

The study was carried out within the framework of the strategic project "Biomedical materials and bioengineering," supported by the program of the Ministry of Education and Science of Russia "Priority 2030" (national project "Science and Universities"). The results of the work are presented in the international scientific journal Applied Surface Science.

2021

Titanium oxide-based multicomponent coating modified with calcium, phosphorus, silicon and boron

On December 7, 2021, NUST MISIS reported that its scientists had developed an antibacterial coating for implants to prevent their rejection and the development of infection under the influence of antibiotic-resistant bacteria. The developed material has high biocompatibility and can find wide application, including in implantology and craniofacial surgery. The study is published in the journal Applied Surface Science.

Principle of antibacterial coating operation

Although implantation has shown good results in the early postoperative period in recent years, the risks of long-term complications associated with the development of inflammation of the tissues surrounding the osteointegrated implant still persist.

The most likely reason for the development of such inflammations may be the penetration of infection into the zone of contact of the implant with the bone. Antibiotic-resistant bacteria such as E. coli and Staphylococcus aureus are particularly dangerous. The solution to the problem of secondary inflammatory complications can be antibacterial coatings for implants.

However, the creation of antibacterial yet biocompatible and bioactive surfaces is a problem that the scientific community has been addressing over the years. Materials that fully meet all these criteria have not yet been synthesized.

Scientists of NUST "MISIS" have created a multicomponent coating based on titanium oxide, allowing to prevent the development of bacterial infection. The coating is further modified with calcium, phosphorus, silicon and boron by arc oxidation, which increases bioactivity of the material. Copper and silver particles were then introduced into the resulting material by ion implantation.

The coating synthesized by Russian scientists has an optimal structure, and modification with bioactive elements allows for more effective osteointegration. In addition, such a coating contributes to the mineralization of adjacent bone tissue.

The results of in vitro studies showed high cellular compatibility and demonstrated 100% bactericidal efficacy against antibiotic-resistant bacterial strains of E. coli and Staphylococcus aureus. The addition of boron particles made it possible to achieve an enhanced biocidal effect. According to the developers, such a coating can find application, including in implantology and craniofacial surgery.

Anastasia Popova

"The relevance of the development is explained by the increasing need for bone implant arthroplasty surgeries associated with an aging population and a wider spread of degenerative diseases. Titanium and its alloys at the beginning of December 2021 are the most widely used materials for replacing damaged areas of bone tissue. But titanium has neither bioactivity nor antibacterial activity. It is known that the process of osteointegration depends, among other things, on factors such as the chemical composition of the surface and its morphology. It is also known that infectious complications in endoprosthesis are one of the significant medical and socio-economic problems, the way to solve which can be a set of measures to prevent the formation of a biofilm on the surface of the implant, "- explains one of the co-authors of the study Anastasia Popova, a 2nd year student of the master's degree program iPhD "Biomaterial Science" NUST "MISIS," an employee of the Scientific and Educational Center for Self-Propagating High-Temperature Synthesis of ISMAN.

The work was carried out within the framework of the international scientific project Russia India-,. financed RUSSIAN FEDERAL PROPERTY FUND

Multilayer antibacterial coating based on titanium oxide and antiseptic components

Young scientists of NUST "MISIS" presented multilayer antibacterial coatings with a prolonged effect and a universal spectrum of action. The coating is based on modified titanium oxide and several antiseptic components. Coatings can be used in implantology as a protective layer for the prevention of concomitant complications - inflammation or rejection of implants. The university announced this on April 16, 2021.

Antibacterial coatings for April 2021 are being actively investigated, as the relevance of the search for alternatives to traditional antibiotics is growing. They can be applied to implants, thereby preventing inflammation caused by nosocomial infections.

However, the creation of antibacterial yet biocompatible and bioactive surfaces is a problem that the scientific community has been addressing over the years, and "dream materials" have never been developed.

Young scientists from the Inorganic Nanomaterials laboratory at NUST MISIS have created an innovative multilayer coating that synthesizes the protective properties of nanoparticles, biopolymers, anticoagulant and antibiotic. The antibiotic and silver nanoparticles provide an antibacterial effect, and heparin prevents bacterial cells from "sticking" to the tissue surface, which reduces the amount of antibacterial agent required.

The method of producing a multilayer coating is a combination of several technologies: first, using magnetron spraying, a thin bioactive nanostructured coating of TiCaPCON was obtained, then silver particles were introduced into the coating by ion implantation, then a biopolymer layer was applied, which in the preparation plays the role of a carrier for bactericidal heparin and gentamicin molecules. - said the author of the work, researcher at the laboratory "Inorganic nanomaterials" NUST "MISIS" Elizaveta Permyakova.

The chemical composition of the obtained coating layers was carefully investigated by the developers using infrared and X-ray photoelectron spectroscopy. It has been found that the introduction of therapeutic components occurs throughout the thickness of the plasma-deposited polymer layer.

Together with colleagues from the State Scientific Center for Applied Microbiology and Biotechnology, scientists studied the influence of each type of antibacterial component (silver ions, gentamicin and heparin) on the antibacterial activity and biocompatibility of the resulting coatings.

In vitro studies of the coating showed cellular compatibility and demonstrated excellent (up to 99%) bactericidal efficacy against the antibiotic-resistant E. Coli bacterial strain.

{{quote 'The combination of several bactericidal fillers and silver ions with a bioactive coating of calcium and phosphorus-modified titanium oxide ensured biocompatibility and a long - up to 7 days - antibacterial effect on the obtained coatings, - Elizaveta Permyakova emphasized. }}

According to the developers, innovative coatings can be used as an antibacterial modifier of the implant, which allows you to speed up its implantation by reducing the risks of concomitant inflammation and stimulating the growth of osteoblastic cells.

For April 2021, researchers plan to move to the preclinical stage of development.

See also

Import substitution of medical devices

Price regulation of medical devices in Russia

Export of medical devices from Moscow

Export of medical devices from Russia

Brain implant

Breast implants

Dental implants

Buttock implants

Spondylodesis implants (global market)

Heart Implants (Global Market)

Bone implantation